In Vitro Evaluation of Candida albicans Adhesion on Heat-Cured Resin-Based Dental Composites.

Microbial adhesion on dental restorative materials may jeopardize the restorative treatment long-term outcome. The goal of this in vitro study was to assess Candida albicans capability to adhere and form a biofilm on the surface of heat-cured dental composites having different formulations but subjected to identical surface treatments and polymerization protocols. Three commercially available composites were evaluated: GrandioSO (GR), Venus Diamond (VD) and Enamel Plus HRi Biofunction (BF). Cylindrical specimens were prepared for quantitative determination of C. albicans S5 planktonic CFU count, sessile cells CFU count and biomass optical density (OD570 nm). Qualitative Concanavalin-A assays (for extracellular polymeric substances of a biofilm matrix) and Scanning Electron Microscope (SEM) analyses (for the morphology of sessile colonies) were also performed. Focusing on planktonic CFU count, a slight but not significant reduction was observed with VD as compared to GR. Regarding sessile cells CFU count and biomass OD570 nm, a significant increase was observed for VD compared to GR and BF. Concanavalin-A assays and SEM analyses confirmed the quantitative results. Different formulations of commercially available resin composites may differently interact with C. albicans. The present results showed a relatively more pronounced antiadhesive effect for BF and GR, with a reduction in sessile cells CFU count and biomass quantification.

Effect of the Abutment Rigidity on the Wear Resistance of a Lithium Disilicate Glass Ceramic: An In Vitro Study.

Lithium disilicate (LDS) glass ceramics are among the most common biomaterials in conservative dentistry and prosthodontics, and their wear behavior is of paramount clinical interest. An innovative in vitro model is presented, which employs CAD/CAM technology to simulate the periodontal ligament and alveolar bone. The model aims to evaluate the effect of the abutment rigidity on the wear resistance of the LDS glass ceramic. Two experimental groups (LDS restorations supported by dental implants, named LDS-on-Implant, or by hybrid ceramic tooth replicas with artificial periodontal ligament, named LDS-on-Tooth-Replica) and a control group (LDS-Cylinders) were compared. Fifteen samples (n = 15) were fabricated for each group and subjected to testing, with LDS antagonistic cusps opposing them over 120,000 cycles using a dual axis chewing simulator. Wear resistance was analyzed by measuring the vertical wear depth (mm) and the volume loss (mm3) on each LDS sample, as well as the linear antagonist wear (mm) on LDS cusps. Mean values were calculated for LDS-Cylinders (0.186 mm, 0.322 mm3, 0.220 mm, respectively), LDS-on-Implant (0.128 mm, 0.166 mm3, 0.199 mm, respectively), and LDS-on-Tooth-Replica (0.098 mm, 0.107 mm3, 0.172 mm, respectively) and compared using one-way-ANOVA and Tukey's tests. The level of significance was set at 0.05 in all tests. Wear facets were inspected under a scanning electron microscope. Data analysis revealed that abutment rigidity was able to significantly affect the wear pattern of LDS, which seems to be more intense on rigid implant-abutment supports compared to resilient teeth replicas with artificial periodontal ligament.

In Vitro Mechanical Properties of a Novel Graphene-Reinforced PMMA-Based Dental Restorative Material.

Recent studies suggest that the incorporation of graphene in resin-based dental materials might enhance their mechanical properties and even decrease their degree of contraction during polymerization. The present study aimed at comparing the three-point flexural strength (FS), the compressive strength (CS), and the Vickers hardness (VH) of a CAD/CAM poly-methylmethacrylate (PMMA)-based resin, a recently introduced graphene-reinforced CAD/CAM PMMA-based resin (G-PMMA), and a conventional dental bis-acryl composite resin (BACR). No significant differences (p > 0.05) were detected among the materials in terms of flexural strength. On the other hand, a mean flexural modulus value of 9920.1 MPa was recorded in BACR group, significantly higher compared to the flexural modulus detected for G-PMMA (2670.2 MPa) and for conventional PMMA (2505.3) (p < 0.05). In terms of compressive modulus (MPa) and compressive strength (MPa), BACR was significantly stiffer than PMMA and G-PMMA. Concerning VH measurements, a significantly increased hardness emerged comparing the BACR group (VH 98.19) to both PMMA and G-PMMA groups (VH 34.16 and 34.26, respectively). Based on the finding of the present study, the graphene-reinforced (PMMA)-based polymer herein tested was not superior to the conventional PMMA and seemed not able to be considered as an alternative material for permanent restorations, at least in terms of hardness and mechanical response to compressive stress. More research on the mechanical/biological properties of G-PMMAs (and on graphene as a filler) seems still necessary to better clarify their potential as dental restorative materials.

Safe and Ultraconservative Rehabilitation of Worn Teeth Patients: How Sectional Clear Aligners May Enhance the Prosthetic Treatment Plan.

This study describes the clinical workflow for occlusal vertical dimension (OVD) increase in patients showing extensive tooth wear and mild teeth misalignment/crowding. A patient affected by dental erosion and occlusal abnormalities was treated to improve her situation. After ascertaining sound condyle and temporomandibular joint (TMJ) conditions, an OVD increase was sought to provide enough inter-occlusal space for the restorations. The use of TMJ three-dimensional imaging throughout the clinical procedures allowed to objectively track the condyle and disk position and confirm a steady condyle-glenoid cavity relationship before definitive restorations placement. Sectional clear aligner therapy prior to totally additive prosthetic rehabilitation allowed obtaining slight derotation and movements of anterior teeth, thus maximizing sound tissue preservation. Adhesively luted restorations were finally delivered on both anterior and posterior sectors. At the end of the treatment, the pre-operative TMJ balance appeared successfully preserved, and the patient was satisfied with the aesthetic and function achieved.

Wear Properties of Conventional and High-Translucent Zirconia-Based Materials.

This study investigated the two-body wear resistance of a first generation 3 mol% yttria-stabilized tetragonal zirconia polycrystal (3Y-TZP), a second generation 3Y-TZP, a third generation 4 mol% yttria partially stabilized zirconia (4Y-PSZ), a 5 mol% yttria partially stabilized zirconia (5Y-PSZ), and a type III gold alloy (Aurocast 8), performed using opposing antagonistic cusps made out of the same material. Eight cylindrical specimens were prepared for each material (n = 8) for a total of forty specimens (N = 40). Conical cusps were fabricated for each material. Each cylinder−cusp pair was arranged inside a two-axis chewing simulator over up to 360,000 loading cycles. The wear resistance was analyzed by measuring the vertical substance loss (mm) and the volume loss (mm3). The antagonist wear (mm) was recorded before and after the wear test to evaluate the linear difference. Statistical analysis was performed using one-way analysis of variance (ANOVA); multiple comparisons were performed according to Tukey's method. No statistically significant differences (p > 0.05) among the first generation 3Y-TZP, second generation 3Y-TZP, and 4Y-PSZ wear were found. 5Y-PSZ showed statistically significant higher wear compared to other the zirconias. Aurocast 8 displayed the highest values in terms of vertical wear, antagonist cusp wear, and volumetric loss. Although still not statistically comparable, the wear behavior of the latest 5Y-PSZ was the closest to the widely recognized gold standard represented by the type III gold alloy.

Meta-analytical analysis on components released from resin-based dental materials.

OBJECTIVES: Resin-based materials are applied in every branch of dentistry. Due to their tendency to release substances in the oral environment, doubts have been raised about their actual safety. This review aims to provide a comprehensive analysis of the last decade literature regarding the concentrations of elutable substances released from dental resin-based materials in different type of solvents. MATERIALS AND METHODS: All the literature published on dental journals between January 2010 and April 2022 was searched using international databases (PubMed, Scopus, Web of Science). Due to strict inclusion criteria, only 23 papers out of 877 were considered eligible. The concentration of eluted substances related to surface and volume of the sample was analyzed, considering data at 24 h as a reference. The total cumulative release was examined as well. RESULTS: The most eluted substances were HEMA, TEGDMA, and BPA, while the less eluted were Bis-GMA and UDMA. Organic solvents caused significantly higher release of substances than water-based ones. A statistically significant inverse correlation between the release of molecules and their molecular mass was observed. A statistically significant positive correlation between the amount of released molecule and the specimen surface area was detected, as well as a weak positive correlation between the release and the specimen volume. CONCLUSIONS: Type of solvent, molecular mass of eluates, and specimen surface and volume affect substances release from materials. CLINICAL RELEVANCE: It could be advisable to rely on materials based on monomers with a reduced elution tendency for clinical procedures.

Safe clinical technique for increasing the occlusal vertical dimension in case of erosive wear and missing teeth.

A safe method to increase the occlusal vertical dimension (OVD) in patients with mild temporomandibular symptoms (such as tenderness upon palpation) is described. After a temporomandibular joint magnetic resonance (MR), an OVD increase was sought, pursuing pure rotational movement without condyle displacement. Prior to definitive rehabilitation, an additional MR confirmed steady and healthy condyle-disc-fossa relationships.

Shear bond strength of glass ionomer and resin-based cements to different types of zirconia.

OBJECTIVE: To investigate the shear bond strength (SBS) of a glass ionomer (GI) cement, an 10-methacryloyloxy-decyl dihydrogen phosphate (MDP)-based adhesive resin cement (MDP-based AC), an MDP-based self-adhesive resin cement (MDP-based SAC), an MDP-free self-adhesive resin cement (MDP-free SAC), and a resin-modified GI (RMGI) cement to a 3 mol% yttria-stabilized (3Y-TZP) and a 5 mol% yttria partially stabilized zirconia (5Y-PSZ). MATERIALS AND METHODS: Fifty blocks were produced using 3Y-TZP and 5Y-PSZ, assigned to subgroups based on the five cements investigated (n = 10) and luted to cylindrical specimens of the same substrate. Each specimen was loaded in a SBS apparatus to failure. Mean SBS (MPa) values and standard deviations were calculated. Data were analyzed using a two-way analysis of variance and Tukey tests (α = .05). Failed specimens were subjected to fractographic analysis. RESULTS: MDP-based AC and MDP-based SAC cements displayed the highest SBS values with both the substrates; GI cement showed the lowest. RMGI and MDP-free SAC cements performed better with 3Y-TZP than with 5Y-PSZ. Fractographic analysis revealed only adhesive and mixed failures. CONCLUSIONS: MDP-based AC and MDP-based SAC cements are suitable for both 3Y-TZP and 5Y-PSZ. MDP-free SAC and RMGI cements are adequate choices for 3Y-TZP, but seem less effective with 5Y-PSZ. CLINICAL SIGNIFICANCE: MDP-based SACs appear to be as reliable as MDP-based ACs for both 3Y-TZP and 5Y-PSZ cementation. Specifically, for 5Y-PSZ cementation, resin-based MDP-free SACs do not seem to guarantee predictable results in terms of SBS.